HPLC-ESI-MS/MS of Imidazole Alkaloids in Pilocarpus microphyllus

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HPLC-ESI-MS/MS of imidazole alkaloids in Pilocarpus microphyllus.

Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC ...

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Seasonal variation in the essential oil of Pilocarpus microphyllus Stapf.

The essential oils of the leaves and fine stems of Pilocarpus microphyllus, collected on iron mineralized soil of the Serra de Caraj s, Southeast of Par State, Brazil, during the rainy and dry seasons, were obtained by hydrodistillation and analyzed by GC-MS. The main identified compounds were 2-tridecanone, beta-caryophyllene, 2-pentadecanone, caryophyllene oxide and germacrene D. Their perce...

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Seasonal change in main alkaloids of jaborandi (Pilocarpus microphyllus Stapf ex Wardleworth), an economically important species from the Brazilian flora

Pilocarpus microphyllus Stapf ex Wardleworth (jaborandi, Rutaceae) is one of the most important Brazilian medicinal species owing to its content of pilocarpine (PIL), an alkaloid used for treating glaucoma and xerostomia. This species contains another alkaloid, epiisopiloturine (EPI), which has demonstrated effectiveness against schistosomiasis. The aim of this work was to assess seasonal chang...

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Genetic diversity of Pilocarpus microphyllus accessions using leaf traits

Among plants with proven medicinal properties, jaborandi (Pilocarpus microphyllus Stapf.) is ascribed priority in conservation programs (Vieira 1999). It is a Rutaceae shrubby tree that occurs in penumbra areas and, according to Skorupa (2000), is found in parts of the Amazon region, mainly in the East of the state of Pará, West and North of Maranhão and North of Piauí. Its leaves contain the i...

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Characterization of the variation in the imidazole alkaloid profile of Pilocarpus microphyllus in different seasons and parts of the plant by electrospray ionization mass spectrometry fingerprinting and identification of novel alkaloids by tandem mass spectrometry.

Pilocarpus microphyllus (Rutaceae), popularly known as jaborandi, is the only commercial source of an imidazole alkaloid named pilocarpine. In the present study, the variation in the profile of imidazole alkaloids in different seasons and in different parts of the P. microphyllus plant during the summer was analyzed by electrospray ionization mass spectrometry in the positive ion mode [ESI(+)-M...

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ژورنال

عنوان ژورنال: Molecules

سال: 2008

ISSN: 1420-3049

DOI: 10.3390/molecules13071518